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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 183
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Cell_line_name |
2HX-2
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Brief_description |
confirmed as mouse with IEF of AST, NP, PEP B established by fusion of SP2/0-
AG14 myeloma cells with spleen cells from BALB/c mice immunized with HELA cells
that were infected with adenovirus 2; cells secrete an IgG monoclonal antibody
reacting with an adenovirus group specific antigen; cell line is patented
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Description |
mouse hybridoma (anti-adenovirus 2) established by fusion of SP2/0-AG14 myeloma
cells with spleen cells from BALB/c mice immunized with HELA cells that were
infected with adenovirus 2; cells secrete an IgG monoclonal antibody reacting with an
adenovirus group specific antigen; cell line is patented confirmed as mouse with IEF of
AST, NP, PEP B
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Depositor |
Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
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Morphology |
single cells growing in suspension
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Culture_conditions |
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS optimal split ratio of 1:2 every 2-
3 days at 37C with 5% CO2 cell harvest of about 1-2 x 106 cells/ml
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Viruses |
ELISA: reverse transcriptase negative; PCR: SMRV -
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Properties |
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule
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Mycolasma |
negative in DAPI, microbiological culture, RNA hybridization assays
Cytogenetics murine karyotype with centric fusion and 16% polyploidy - 66(58-74)<3n>
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Bibliographic_reference |
Cepko et al., Virology, 110: 17 (1981) [PMID: 7222481
] |